integrate.yaml Reference

This configuration file corresponds to --option=integrate and defines how input data are organized for ingestion and cross-sample integration. The default values are listed below.

Parameter

Default

Description

option

integrate

Fixed identifier for the analysis stage

results_folder

results

Root directory for analysis outputs

data_fold

data

Root directory for raw input data

sample_list

sample.txt

Path to the sample list file

run_type

visium

Platform type, such as visium, visium_HD, or xenium

channel

compare_analysis

Analysis channel, either single-sample or multi-sample

cells_boundaries

False

Whether to load the cell boundary layer for Xenium

nucleus_boundaries

False

Whether to load the nucleus boundary layer for Xenium

nucleus_labels

False

Whether to load the nucleus label layer for Xenium

morphology_mip

False

Whether to load the morphology MIP image for Xenium

geojson

cell_segmentations.geojson

Segmentation filename for visium_segment

image

tissue_hires_image.png

Tissue image filename for visium_segment

scale_factors

scalefactors_json.json

Scale factor filename

coor_file

BeadLocationsForR.csv

Coordinate filename for slide_seq

Tuning suggestions

  1. In most projects, you only need to modify run_type, channel, and sample_list.

  2. Enable or adjust layer-related parameters only for Xenium or visium_segment data.

  3. In collaborative projects, keep results_folder fixed so that downstream paths remain stable and reproducible.